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99%+ HPLC Verified Purity

TB-500 Peptide: High-Purity Research Grade Compound

Category

Regenerative Research

CAS Number

77591-33-4

Form Factor

Lyophilized Powder

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TB-500 is a synthetic 43-amino-acid peptide investigated as a functional analogue of Thymosin Beta-4. Experimental research examines its interactions with actin-polymerization pathways and associated effects on cellular migration and tissue remodeling. Additional study endpoints include inflammatory signaling, apoptosis regulation, and hair-follicle activity.

TB-500 is a synthetic 43-amino-acid peptide investigated as a functional analogue of Thymosin Beta-4. Experimental research examines its interactions with actin-polymerization pathways and associated effects on cellular migration and tissue remodeling. Additional study endpoints include inflammatory signaling, apoptosis regulation, and hair-follicle activity.

CAS Number

77591-33-4

Secondary CAS

885340-08-9

Purity

99%+ HPLC Verified

Molecular Formula

C212H350N56O78S

Molar Mass

4963.4421 g/mol

Form Factor

Lyophilized Powder

Synonyms

Thymosin β-4 Fragment TB4

Storage Conditions

Unreconstituted

Store at -20°C to 4°C (freezer or refrigerator). Stable for 24+ months.

Reconstituted

Refrigerate at 2–8°C. Use within 30 days for optimal stability.

Avoid

Repeated freeze-thaw cycles, direct sunlight, and temperatures above 25°C.

Safety & Compliance

  • Wear nitrile gloves and safety goggles when handling research compounds.
  • Work in a clean, controlled environment (laminar flow hood recommended).
  • Dispose of sharps and used materials in approved biohazard containers.
  • This product is for laboratory research use only (RUO) — not for human or veterinary use.

Quality Assurance Standards

Each production lot is subjected to rigorous analytical evaluation to meet pharmaceutical-grade specifications. Full testing results and procedural documentation are furnished through the accompanying Certificate of Analysis (COA) with every shipment.

Certificate of Analysis

Latest COA for TB-500 — independently tested via HPLC and Mass Spectrometry.

Testing Methodology

Every batch undergoes a multi-stage analytical pipeline before release. Results are compiled into the Certificate of Analysis (COA) included with each order.

  • • HPLC — High-Performance Liquid Chromatography confirms peptide purity ≥98%.
  • • Mass Spectrometry — Validates molecular identity and correct sequence.
  • • Endotoxin (LAL) — Limulus Amebocyte Lysate assay ensures endotoxin levels are within safe limits.
  • • Sterility — Microbial contamination testing per USP standards.

Learn More About Peptides

Explore our research library covering purity, synthesis, storage, solubility, and more.

Peptide Information

Research Database

Browse peer-reviewed studies, clinical trials, and scientific sources behind our peptide resources.

View Research

Why Research Labs

  • Buy With Confidence

    COA links, HPLC data, and transparent pricing on every product page.

  • Fair Pricing, Higher Standards

    Transparent, competitive pricing without compromising the quality standards behind every batch.

  • Independently Tested

    Verified at 99%+ purity and sterility by third-party labs.

  • Shipped Same Day

    Order by 12 PM PT for guaranteed same-day shipping and tracking.

Complementary Research Compounds and Peptides

TB-500 is a synthetic 43-amino-acid peptide investigated as a functional analogue of Thymosin Beta-4. Experimental research examines its interactions with actin-polymerization pathways and associated effects on cellular migration and tissue remodeling. Additional study endpoints include inflammatory signaling, apoptosis regulation, and hair-follicle activity.

CAS Number

77591-33-4

Secondary CAS

885340-08-9

Purity

99%+ HPLC Verified

Molecular Formula

C212H350N56O78S

Molar Mass

4963.4421 g/mol

Form Factor

Lyophilized Powder

Synonyms

Thymosin β-4 Fragment TB4

Storage Conditions

Unreconstituted

Store at -20°C to 4°C (freezer or refrigerator). Stable for 24+ months.

Reconstituted

Refrigerate at 2–8°C. Use within 30 days for optimal stability.

Avoid

Repeated freeze-thaw cycles, direct sunlight, and temperatures above 25°C.

Safety & Compliance

  • Wear nitrile gloves and safety goggles when handling research compounds.
  • Work in a clean, controlled environment (laminar flow hood recommended).
  • Dispose of sharps and used materials in approved biohazard containers.
  • This product is for laboratory research use only (RUO) — not for human or veterinary use.

Quality Assurance Standards

Each production lot is subjected to rigorous analytical evaluation to meet pharmaceutical-grade specifications. Full testing results and procedural documentation are furnished through the accompanying Certificate of Analysis (COA) with every shipment.

Certificate of Analysis

Latest COA for TB-500 — independently tested via HPLC and Mass Spectrometry.

Testing Methodology

Every batch undergoes a multi-stage analytical pipeline before release. Results are compiled into the Certificate of Analysis (COA) included with each order.

  • • HPLC — High-Performance Liquid Chromatography confirms peptide purity ≥98%.
  • • Mass Spectrometry — Validates molecular identity and correct sequence.
  • • Endotoxin (LAL) — Limulus Amebocyte Lysate assay ensures endotoxin levels are within safe limits.
  • • Sterility — Microbial contamination testing per USP standards.

Learn More About Peptides

Explore our research library covering purity, synthesis, storage, solubility, and more.

Peptide Information

Research Database

Browse peer-reviewed studies, clinical trials, and scientific sources behind our peptide resources.

View Research

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Frequently Asked Questions (RUO Compliance)

1. What is the structural relationship between TB-500 and Thymosin Beta-4?

TB-500 is a synthetic peptide fragment representing the active sequence Ac-Leu-Lys-Lys-Thr-Glu-Thr-Gln-OH (LKKTETQ), corresponding to the primary actin-binding domain of endogenous Thymosin Beta-4. It possesses a molecular formula of C38H68N10O14 and molecular weight of 888.99 g/mol.

2. What is the primary cellular mechanism studied with TB-500 in research?

TB-500 is evaluated in preclinical cellular models for actin sequestration dynamics, regulation of cell motility and migration, stimulation of angiogenesis via endothelial cell sprouting, and dermal matrix remodeling pathways.

3. What is the certified chromatographic purity of TB-500?

Each batch of TB-500 is analytical-grade with chromatographic purity verified at >=99.0% using reversed-phase High-Performance Liquid Chromatography (RP-HPLC) and sequence-confirmed via electrospray ionization mass spectrometry (ESI-MS).

4. How should lyophilized TB-500 be stored upon receipt?

Lyophilized vials should be maintained at -20°C in a desiccated, light-protected storage unit to preserve long-term chemical stability. Short-term ambient transit (<25°C) does not induce degradation.

5. What liquid diluent is recommended for laboratory reconstitution?

Reconstitution is typically performed using sterile bacteriostatic water (containing 0.9% benzyl alcohol) or sterile 0.9% sodium chloride for laboratory assays, providing antimicrobial stability during multiple analytical sampling procedures.

6. What is the proper reconstitution technique to prevent peptide damage?

Introduce the diluent slowly along the inner glass wall of the vial under aseptic conditions. Avoid forceful direct jet streams onto the lyophilized powder cake, and swirl gently without vortexing or vigorous shaking to protect tertiary molecular structure.

7. What are the storage requirements for reconstituted TB-500 solutions?

Following reconstitution, the liquid peptide solution must be stored strictly refrigerated at 2°C to 8°C, shielded from UV and direct light, and utilized within 28–30 days for maximum experimental reproducibility.

8. Can reconstituted TB-500 liquid be refrozen?

Repeated freeze-thaw cycles of reconstituted liquid solutions should be avoided, as ice crystal formation can promote peptide aggregation and cleavage of the amide backbone.

9. How can researchers access the Certificate of Analysis (COA)?

Batch-specific Certificates of Analysis (COA) containing analytical HPLC chromatograms, mass spectral confirmation, and net peptide content are verifiable and downloadable from the Research Labs verification portal using the lot identifier.

10. What is the legal and regulatory compliance status of TB-500?

TB-500 is synthesized and distributed exclusively for in vitro scientific research, academic experimentation, and laboratory evaluation by certified professionals (Research Use Only). It is strictly prohibited for human, medical, diagnostic, veterinary, or athletic performance use.

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